The effect of frequency in the electrical stimulation of chondrocytes

El efecto de la frecuencia en la estimulación eléctrica de condrocitos

  • Juan Jairo Vaca González Fundación Universitaria del Área Andina
  • Juan Felipe Escobar Huertas Fundación Universitaria Los Libertadores
  • Diego Alexander Garzón Alvarado Universidad Nacional de Colombia
Palabras clave: Cartilage Explants, Chondrocytes, Computational model, Electric Fields, Frequency Dependence, Scaffolds (en_US)
Palabras clave: Explantes de cartílago, Condrocitos, Modelo Computacional, Campos Eléctricos, Dependencia de la Frecuencia, Andamios (es_ES)

Resumen (en_US)

Electrical stimulation is a non-invasive therapy used to stimulate chondrocyte dynamics: proliferation, migration, morphology and molecular synthesis. Some studies have evidenced the role of frequency in the generation of electric fields; however, the electrical stimulation sensed by chondrocytes when the frequency varies is not well-documented. Accordingly, a computational model was implemented to assess the frequency dependence of electric fields that stimulate chondrocytes. Cells were modelled in three different scenarios: monolayer cultures, cartilage explants and scaffolds. Chondrocytes were stimulated with 100 Vp-p at frequencies of 0.001, 1, 10, 50, 100 and 1000 kHz. Results showed that frequency is a relevant factor when considering the stimulation of biological samples, since electric fields increased as frequencies were higher. Moreover, chondrocytes experienced different electric fields in both cytoplasm and extracellular environment. This model provides relevant information about the electrical parameters to stimulate cells; in fact, it could enhance experimental procedures, predicting the stimulation that improves chondrocyte dynamics. Electric fields are a promising tool to maintain either well-structured chondrocytes or biomimetic materials used in regenerative therapies such as autologous implantation to treat hyaline cartilage injuries.

Resumen (es_ES)

La estimulación eléctrica es una terapia no invasiva utilizada para estimular la dinámica de los condrocitos: proliferación, migración, morfología y síntesis molecular. Algunos estudios han evidenciado el rol de la frecuencia en la generación de campos eléctricos; sin embargo, la estimulación eléctrica percibida por el condrocito cuando la frecuencia varia no está bien documentada. Por esto, se implementó un modelo computacional para evaluar la dependencia de la frecuencia de los campos eléctricos que estimulan los condrocitos. Las células fueron modeladas en tres escenarios diferentes: cultivos monocapa, explantes de cartílago y andamios. Los condrocitos fueron estimulados con 100 Vp-p a frecuencias de 0.001, 1, 10, 50, 100 y 1000 kHz. Los resultados mostraron que la frecuencia es un factor relevante para estimular muestras biológicas, ya que los campos eléctricos aumentaron a medida que la frecuencia se incrementó. Además, los condrocitos experimentaron diferentes campos eléctricos tanto en el citoplasma como en el ambiente extracelular. Este modelo provee información relevante acerca de los parámetros eléctricos para estimular células; de hecho, este puede mejorar los procedimientos experimentales, prediciendo la estimulación que mejora la dinámica de los condrocitos. Los campos eléctricos son una herramienta prometedora para mantener ya sea condrocitos bien caracterizados o materiales biomiméticos usados en terapias regenerativas tales como la implantación autóloga para tratar lesiones del cartílago hialino.


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Cómo citar
Vaca González, J. J., Escobar Huertas, J. F., & Garzón Alvarado, D. A. (2020). El efecto de la frecuencia en la estimulación eléctrica de condrocitos. Visión electrónica, 14(1). Recuperado a partir de
Publicado: 2020-01-31
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