DOI:

https://doi.org/10.14483/23448350.17538

Publicado:

2021-05-01

Número:

Vol. 41 Núm. 2 (2021): mayo-agosto

Sección:

Ciencia y Tecnología

Genetic Detection of Neospora caninum in the Blood of Dairy Cattle from Boyacá, Colombia

Detección genética de Neospora caninum en sangre de ganado lechero de Boyacá, Colombia

Autores/as

  • Tania-Alejandra Correa-Castro Universidad Pedagógica y Tecnológica de Colombia https://orcid.org/0000-0003-0494-7587
  • Andrés-Mauricio Gómez-Palacio Universidad Pedagógica y Tecnológica de Colombia https://orcid.org/0000-0002-1069-9199
  • Martin-Orlando Pulido-Medellín Universidad Pedagógica y Tecnológica de Colombia

Palabras clave:

detección molecular, ganado lechero, genética, neosporosis, PCR anidada (es).

Palabras clave:

dairy cattle, genetics, molecular detection, nested PCR, neosporosis (en).

Descargas

Resumen (en)

Neospora caninum is a parasite of the phylum Apicomplexa that causes significant economic losses for cattle husbandry worldwide. Despite its relevance, information regarding infection prevalence in endemic areas of Colombia is scarce. Previous studies have reported a high seroprevalence in dairy cattle from Boyacá, which suggests a significant risk factor for active transmission of neosporosis. However, there is no available data concerning the infection stage or the presence of said parasite in the peripheral blood of dairy cattle. In this study, genetic detection of N. caninum was carried out using a nested PCR with an Nc-5 marker on peripheral blood samples from dairy cows in the municipalities of Paipa, Toca, and Tuta. Four positive samples were sequenced through the Sanger method, which were then edited, aligned, and compared to sequences available in the GenBank database. The parasite’s DNA was detected in 23 out of 170 analyzed blood samples. A qualitative detection limit was estimated (~64 parasites per volume of blood sampled). This is the first report of DNA detection of N. caninum through PCR in blood from Colombian dairy cattle. Thereupon, further studies about N. caninum molecular detection and population genetics in cattle peripheral blood could be very useful for the early diagnosis of neosporosis and creating more effective epidemiological surveillance strategies.

Resumen (es)

Neospora caninum es un parásito del filo Apicomplexa que genera grandes pérdidas económicas para la ganadería a nivel mundial. A pesar de su importancia, la información sobre la prevalencia de infección en áreas endémicas de Colombia es escasa. Estudios previos han reportado una alta seroprevalencia en ganado lechero de Boyacá, que sugiere un factor de riesgo significativo para la transmisión activa de la neosporosis. Sin embargo, no hay datos disponibles sobre el estado de infección o la presencia del parásito en la sangre periférica del ganado lechero. En este estudio, se realizó la detección genética de N. caninum por PCR anidada del marcador Nc-5 en muestras de sangre periférica de vacas lecheras de los municipios de Paipa, Toca y Tuta. Se secuenciaron cuatro muestras positivas por el método de Sanger, que se editaron, alinearon y compararon con secuencias disponibles en la base de datos GenBank. Se detectó el ADN del parásito en 23 de las 170 muestras de sangre analizadas. Se estimó un límite cualitativo de detección en las muestras (~64 parásitos por volumen de sangre muestreada). Este es el primer reporte de detección del ADN de N. caninum por PCR en sangre de ganado lechero colombiano. Por lo tanto, futuros trabajos sobre la detección molecular y la genética poblacional de N. caninum en sangre periférica de ganado podrían ser muy útiles para el diagnóstico temprano de la neosporosis y la creación de estrategias más efectivas de vigilancia epidemiológica.

Referencias

Álvarez-García, G., Collantes-Fernández, E., Costas, E., Rebordosa, X., Ortega-Mora, L. M. (2003). Influence of age and purpose for testing on the cut-off selection of serological methods in bovine neosporosis. Veterinary Research, 34(3), 341-352. https://doi.org/10.1051/vetres:2003009

Amdouni, Y., Rjeibi, M. R., Awadi, S., Rekik, M., Gharbi M. (2018). First detection and molecular identification of Neospora caninum from naturally infected cattle and sheep in North Africa. Transboundary and Emerging Diseases, 65(4), 976-982. https://doi.org/10.1111/tbed.12828

Amdouni, Y., Rouatbi, M., Lassoued, N., Rekik, M., Gharbi, M. (2019). Neospora caninum Natural Infection in Tunisian Rams: Serological Study and Molecular Identification of Infection in Semen. Acta Parasitologica, 64, 821-828. https://doi.org/10.2478/s11686-019-00105-0

Bărburaș, D., Gyӧrke, A., Ionică, A. M., Bărburaș, R., Mircean, V., Cozma, V. (2019). Evidence of Neospora caninum infection in buffaloes (Bubalus bubalis) from Northwestern Romania. Parasitolology Research, 118, 1667-1671. https://doi.org/10.1007/s00436-019-06263-4

Cardona, J. A., Martínez, Y., Betancur, C. A. (2015). Seroepidemiología de hembras bovinas naturalmente infectadas por Neospora caninum en Córdoba, Colombia. Revista UDCA Actualidad & Divulgación Científica, 18(2), 401-408. https://doi.org/10.31910/rudca.v18.n2.2015.166

Changoluisa, D., Rivera-Olivero, I. A., Echeverria, G., Garcia-Bereguiain, M. A., de Waard J. H., Applied Microbiology group of the School of Biological Sciences and Engineering at Yachay University (2019). Serology for Neosporosis, Q fever and Brucellosis to assess the cause of abortion in two dairy cattle herds in Ecuador. BMC Veterinary Research, 15, 194. https://doi.org/10.1186/s12917-019-1924-7

Collantes-Fernández, E., Zaballos, Á., Álvarez-García, G., Ortega-Mora, L. M. (2002). Quantitative detection of Neospora caninum in bovine aborted fetuses and experimentally infected mice by real-time PCR. Journal of Clinical Microbiology, 40(4), 1194-1198. https://doi.org/10.1128/jcm.40.4.1194-1198.2002

Cruz-Estupiñán, S., Díaz-Anaya, A., Bulla-Castañeda, D., García-Corredor, D., Pulido-Medellín, M. (2019). Diagnóstico Serológico de Neospora caninum en vacas del municipio de Tuta, Boyacá. Revista de la Facultad de Medicina Veterinaria y Zootecnia, 66(3), 197-207. https://doi.org/10.15446/rfmvz.v66n3.84256

da Silva, J. B., Nicolino, R. R., Fagundes, G. M., dos Anjos Bomjardim, H., dos Santos Belo Reis, A., da Silva Lima, D. H., Oliveira, C. M. C., Barbosa, J. D., da Fonseca, A. H. (2017). Serological survey of Neospora caninum and Toxoplasma gondii in cattle (Bos indicus) and water buffaloes (Bubalus bubalis) in ten provinces of Brazil. Comparative Immunology, Microbiology and Infectious Diseases, 52, 30-35. https://doi.org/10.1016/j.cimid.2017.05.005

de Magalhães, V. C., de Oliveira, U. V., Costa, S. C., Santos, I. A., Pereira, M. J., Munhoz A. D. (2014). Transmission paths of Neospora caninum in a dairy herd of crossbred cattle in the northeast of Brazil. Veterinary Parasitology, 202(3-4), 257-264. https://doi.org/10.1016/j.vetpar.2014.01.018

Dolezel, J., Bartos, J., Voglmayr, H., Greilhuber, J. (2003). Nuclear DNA content and genome size of trout and human. Journal of Qualitative Cell Science: Cytometry, Part A, 51A(2), 127-129. https://doi.org/10.1002/cyto.a.10013

Dubey, J. P. (2003). Review of Neospora caninum and neosporosis in animals. The Korean Journal of Parasitology, 41(1), 1. https://doi.org/10.3347/kjp.2003.41.1.1

Dubey, J. P., Schares, G. (2006). Diagnosis of bovine neosporosis. Veterinary Parasitology, 140(1-2), 1-34. https://doi.org/10.1016/j.vetpar.2006.03.035

Dubey, J. P., Schares, G., Ortega-Mora, L. M. (2007). Epidemiology and control of neosporosis and Neospora caninum. Clinical Microbiolology Reviews, 20(2), 323-367. https://doi.org/10.1128/CMR.00031-06

Ferre, I., Aduriz, G., del Pozo, I., Regidor-Cerrillo, J., Atxaerandio, R., Collantes-Fernández, E., Hurtado, A., Ugarte-Garagalza, C., Ortega-Mora L. M. (2005). Detection of Neospora caninum in the semen and blood of naturally infected bulls. Theriogenology, 63(5),1504-1518. https://doi.org/10.1016/j.theriogenology.2004.07.002

García-Melo, D. P., Regidor-Cerrillo, J., Ortega-Mora, L. M., Collantes-Fernández, E., de Oliveira, V. S. F., de Oliveira, M. A. P., da Silva A. C. (2009). Isolation and biological characterisation of a new isolate of Neospora caninum from an asymptomatic calf in brazil. Acta Parasitologica, 54(2), 180-185. https://doi.org/10.2478/s11686-009-0018-2

Gondim, L. F., Laski, P., Gao, L., McAllister, M. M. (2004). Variation of the internal transcribed spacer 1 sequence within individual strains and among different strains of Neospora caninum. Journal of Parasitology, 90(1), 119-123. https://doi.org/10.1645/GE-134R

Hall, T. A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95-98.

Instituto Colombiano Agropecuario (ICA) (2017). Censo Pecuario 2017. https://www.ica.gov.co/areas/pecuaria/servicios/epidemiologiaveterinaria/censos-2016/censo-2017.aspx

Klun, I., Ćirković, V., Maletić, M., Bradonjić, S., Djurković-Djaković, O. (2019). Seroprevalence of Neospora caninum infection and associated risk factors in dairy cattle in Serbia. Parasitology Research, 118, 1875-1883. https://doi.org/10.1007/s00436-019-06307-9

Kumar, S., Stecher, G., Li, M., Knyaz, C., Tamura, K. (2018). MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution, 35(6), 1547-1549. https://doi.org/10.1093/molbev/msy096

Li, J., He, P., Yu, Y., Du, L., Gong, P., Zhang, G., Zhang, X. (2014). Detection of Neospora caninum-DNA in feces collected from dogs in Shenyang (China) and ITS1 phylogenetic analysis. Veterinary Parasitology, 205(1-2), 361-364. https://doi.org/10.1016/j.vetpar.2014.06.036

Lista-Alves, D., Palomares-Naveda, R., García, F., Obando, C., Arrieta, D., Hoet, A. E. (2006). Serological evidence of Neospora caninum in dual-purpose cattle herds in Venezuela. Veterinary Parasitology, 13(3-4), 347-349. https://doi.org/10.1016/j.vetpar.2005.11.027

Llano, H. A. B., Guimarães, M. S., Soares, R. M., Polo, G., da Silva, A. C. (2018). Seroprevalence and risk factors for Neospora caninum infection in cattle from the eastern Antioquia, Colombia. Veterinary and Animal Science, 6, 69-74. https://doi.org/10.1016/j.vas.2018.03.001

McInnes, L. M., Ryan, U. M., O'Handley, R., Sager, H., Forshaw, D., Palmer, D. G. (2006). Diagnostic significance of Neospora caninum DNA detected by PCR in cattle serum. Veterinary Parasitology, 142(3-4), 207-213. https://doi.org/10.1016/j.vetpar.2006.07.013

Moore, D. P., Konrad, J. L., San Martino, S., Reichel, M. P., Cano, D. B., Méndez, S., Späth, E. J., Odeón, A. C., Crudeli, G., Campero C. M. (2014). Neospora caninum serostatus is affected by age and species variables in cohabiting water buffaloes and beef cattle. Veterinary Parasitology, 203(3-4), 259-263. https://doi.org/10.1016/j.vetpar.2014.04.011

Motta-Giraldo, J., Waltero-García, I., Abeledo, M., Fernández, O. (2012). Estudio retrospectivo de agentes infecciosos que afectan la reproducción bovina en el departamento del Caquetá, Colombia. Revista de Salud Animal, 34(3), 159-164.

Müller, N., Zimmermann, V., Hentrich, B., Gottstein, B. (1996). Diagnosis of Neospora caninum and Toxoplasma gondii infection by PCR and DNA hybridization immunoassay. Journal of Clinical Microbiology, 34(11), 2850-2852. https://doi.org/10.1128/JCM.34.11.2850-2852.1996

Nardoni, S., Poli, A., Varvaro, I., Rocchigiani, G., Ceccherelli, R., Mancianti, F. (2019). Detection of Neospora Caninum DNA in Wild Birds from Italy. Pathogens, 8(4), 202. https://doi.org/10.3390/pathogens8040202

Okeoma, C. M., Williamson, N. B., Pomroy, W. E., Stowell, K. M., Gillespie, L. (2004). The use of PCR to detect Neospora caninum DNA in the blood of naturally infected cows. Veterinary Parasitology, 122(4), 307-315., https://doi.org/10.1016/j.vetpar.2004.06.001

Pagmadulam, B., Myagmarsuren, P., Fereig, R. M., Igarashi, M., Yokoyama, N., Battsetseg, B., Nishikawa, Y. (2018). Seroprevalence of Toxoplasma gondii and Neospora caninum infections in cattle in Mongolia. Veterinary Parasitology: Regional Studies and Reports, 14, 11-17. https://doi.org/10.1016/j.vprsr.2018.08.001

Gobernación de Boyacá (Secretaría de Agricultura) (2019). Plan Departamental de Extension Agropecuaria. https://www.minagricultura.gov.co/ministerio/direcciones/Documents/PDEA%27s%20Aprobados/PDEA%20Boyac%C3%A1.pdf

Pulido-Medellín, M. O., García-Corredor, D. J., Vargas-Abella, J. C. (2016). Seroprevalencia de Neospora caninum en un Hato Lechero de Boyacá, Colombia. Revista de Investigaciones Veterinarias del Perú, 27(2), 355-362. https://doi.org/10.15381/rivep.v27i2.11658

Ramos, A. E., Muñoz, M., Cortés-Vecino, J. A., Barato, P., Patarroyo M. A. (2017). A novel loop-mediated isothermal amplification-based test for detecting Neospora caninum DNA. Parasites & Vectors, 10(1), 590. https://doi.org/10.1186/s13071-017-2549-y

Regidor-Cerrillo, J., Díez-Fuertes, F., García-Culebras, A., Moore, D. P., González-Warleta, M., Cuevas, C., Schares, G., Katzer, F., Pedraza-Díaz, S., Mezo, M. (2013). Genetic diversity and geographic population structure of bovine Neospora caninum determined by microsatellite genotyping analysis. PLoS One 8(8), e72678. https://doi.org/10.1371/journal.pone.0072678

Reid, A. J., Vermont, S. J., Cotton, J. A., Harris, D., Hill-Cawthorne, G. A., Könen-Waisman, S., Latham, S. M., Mourier, T., Norton, R., Quail, M. A. (2012). Comparative genomics of the apicomplexan parasites Toxoplasma gondii and Neospora caninum: Coccidia differing in host range and transmission strategy. PLoS Pathogens, 8(3), e1002567. https://doi.org/10.1371/journal.ppat.1002567

Rocchigiani, G., Poli, A., Nardoni, S., Papini, R., Mancianti, F. (2017). Neospora caninum in Wild Waterfowl: Occurrence of Parasite DNA and Low Antibody Titers. Journal of Parasitology, 103, 142-145. https://doi.org/10.1645/16-34

Serrano-Martínez, M. E., Cisterna, C. A. B., Romero, R. C. E., Huacho, M. A. Q., Bermabé, A. M., Albornoz, L. A. L. (2019). Evaluation of abortions spontaneously induced by Neospora caninum and risk factors in dairy cattle from Lima, Perú. Revista Brasileira de Parasitologia Veterinária, 28, 215-220. https://doi.org/10.1590/S1984-29612019026

Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F., Higgins, D. G. (1997). The ClustalX windows interface: Xexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 24, 4876-4882. https://doi.org/10.1093/nar/25.24.4876

Villagra-Blanco, R., Angelova, L., Conze, T., Schares, G., Bärwald, A., Taubert, A., Hermosilla, C., Wehrend, A. (2018). Seroprevalence of Neospora caninum-specific antibodies in German breeding bitches. Parasites & Vectors, 11, 96. https://doi.org/10.1186/s13071-018-2683-1

Yao, L., Yang, N., Liu, Q., Wang, M., Zhang, W., Qian, W., Hu, Y., Ding, J. (2009). Detection of Neospora caninum in aborted bovine fetuses and dam blood samples by nested PCR and ELISA and seroprevalence in Beijing and Tianjin, China. Parasitology, 136(11), 1251-1256. https://doi.org/10.1017/S0031182009990813

Yu, J., Xia, Z., Liu, Q., Liu, J., Ding, J., Zhang, W. (2007). Seroepidemiology of Neospora caninum and Toxoplasma gondii in cattle and water buffaloes (Bubalus bubalis) in the People's Republic of China. Veterinary Parasitology, 143(1), 79-85. https://doi.org/10.1016/j.vetpar.2006.07.031

Zambrano, J., Cotrino, V., Jiménez, C., Romero, M., Guerrero, B. (2001). Evaluación serológica de Neospora caninum en bovinos en Colombia. Revista Acovez, 26, 5-10.

Cómo citar

APA

Correa-Castro, T.-A., Gómez-Palacio, A.-M., & Pulido-Medellín, M.-O. (2021). Genetic Detection of Neospora caninum in the Blood of Dairy Cattle from Boyacá, Colombia. Revista Científica, 41(2), 252–263. https://doi.org/10.14483/23448350.17538

ACM

[1]
Correa-Castro, T.-A., Gómez-Palacio, A.-M. y Pulido-Medellín, M.-O. 2021. Genetic Detection of Neospora caninum in the Blood of Dairy Cattle from Boyacá, Colombia. Revista Científica. 41, 2 (may 2021), 252–263. DOI:https://doi.org/10.14483/23448350.17538.

ACS

(1)
Correa-Castro, T.-A.; Gómez-Palacio, A.-M.; Pulido-Medellín, M.-O. Genetic Detection of Neospora caninum in the Blood of Dairy Cattle from Boyacá, Colombia. Rev. Cient. 2021, 41, 252-263.

ABNT

CORREA-CASTRO, T.-A.; GÓMEZ-PALACIO, A.-M.; PULIDO-MEDELLÍN, M.-O. Genetic Detection of Neospora caninum in the Blood of Dairy Cattle from Boyacá, Colombia. Revista Científica, [S. l.], v. 41, n. 2, p. 252–263, 2021. DOI: 10.14483/23448350.17538. Disponível em: https://revistas.udistrital.edu.co/index.php/revcie/article/view/17538. Acesso em: 15 jun. 2021.

Chicago

Correa-Castro, Tania-Alejandra, Andrés-Mauricio Gómez-Palacio, y Martin-Orlando Pulido-Medellín. 2021. «Genetic Detection of Neospora caninum in the Blood of Dairy Cattle from Boyacá, Colombia». Revista Científica 41 (2):252-63. https://doi.org/10.14483/23448350.17538.

Harvard

Correa-Castro, T.-A., Gómez-Palacio, A.-M. y Pulido-Medellín, M.-O. (2021) «Genetic Detection of Neospora caninum in the Blood of Dairy Cattle from Boyacá, Colombia», Revista Científica, 41(2), pp. 252–263. doi: 10.14483/23448350.17538.

IEEE

[1]
T.-A. Correa-Castro, A.-M. Gómez-Palacio, y M.-O. Pulido-Medellín, «Genetic Detection of Neospora caninum in the Blood of Dairy Cattle from Boyacá, Colombia», Rev. Cient., vol. 41, n.º 2, pp. 252–263, may 2021.

MLA

Correa-Castro, T.-A., A.-M. Gómez-Palacio, y M.-O. Pulido-Medellín. «Genetic Detection of Neospora caninum in the Blood of Dairy Cattle from Boyacá, Colombia». Revista Científica, vol. 41, n.º 2, mayo de 2021, pp. 252-63, doi:10.14483/23448350.17538.

Turabian

Correa-Castro, Tania-Alejandra, Andrés-Mauricio Gómez-Palacio, y Martin-Orlando Pulido-Medellín. «Genetic Detection of Neospora caninum in the Blood of Dairy Cattle from Boyacá, Colombia». Revista Científica 41, no. 2 (mayo 1, 2021): 252–263. Accedido junio 15, 2021. https://revistas.udistrital.edu.co/index.php/revcie/article/view/17538.

Vancouver

1.
Correa-Castro T-A, Gómez-Palacio A-M, Pulido-Medellín M-O. Genetic Detection of Neospora caninum in the Blood of Dairy Cattle from Boyacá, Colombia. Rev. Cient. [Internet]. 1 de mayo de 2021 [citado 15 de junio de 2021];41(2):252-63. Disponible en: https://revistas.udistrital.edu.co/index.php/revcie/article/view/17538

Descargar cita

Visitas

54

Dimensions


PlumX


Descargas

Los datos de descargas todavía no están disponibles.