DOI:

https://doi.org/10.14483/23448350.19724

Publicado:

2023-01-02

Número:

Vol. 46 Núm. 1 (2023): Enero-Abril 2023

Sección:

Ciencias Básicas y Educación

Diagnóstico serológico de Fasciola hepatica en ovinos de Boavita (Colombia)

Serological Diagnosis of Fasciola hepatica in Sheeps from Boavita (Colombia)

Autores/as

Palabras clave:

ELISA, Fasciolosis, Enfermedades de las ovejas, Serología, Seroprevalencia (es).

Palabras clave:

ELISA, fasciolosis, seroprevalence serology, sheep diseases (en).

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Resumen (es)

La fasciolosis es una enfermedad de distribución mundial causada por el trematodo Fasciola hepática, que afecta bovinos, ovinos, caprinos y en ocasiones al ser humano, causando importantes pérdidas económicas y productivas. El objetivo de este estudio fue determinar la seroprevalencia de F. hepatica en ovinos de Boavita, Boyacá, Colombia. Se realizó un estudio observacional y descriptivo de corte (transversal) con muestreo aleatorio simple, en donde el tamaño muestral fue de 297 ovinos. Los sueros fueron procesados con el kit comercial ELISA BIO K 211-Monoscreen AbELISA F. hepatica, siguiendo las instrucciones del fabricante. El análisis de los resultados se realizó con el programa estadístico Epi Info™. Se determinó una seroprevalencia general de 67.34 % en donde los machos, los ovinos mayores de 3 años y las ovejas de raza criolla presentaron la seroprevalencia más alta, con 77.78, 83.33 y 64.79% respectivamente. Se estableció asociación estadística significativa entre la seropositividad a F. hepatica y los ovinos de 1 a 3 años y los individuos mayores 3 años. Este grupo etario se estableció como factor de riesgo para la presentación de fasciolosis, mientras que las ovejas entre 1 y 3 años se consideraron como factor de protección. Las variables de manejo como pastoreo extensivo y de estaca también presentaron significancia estadística, siendo este último un factor de protección para la parasitosis. Nuestros hallazgos evidencian la diseminación de la infección por F. hepatica en ejemplares ovinos y confirman la importancia de los métodos serológicos para el diagnóstico de la enfermedad.

Resumen (en)

Fasciolosis is a worldwide disease caused by the trematode Fasciola hepatica, which affects cattle, sheep, goats, and sometimes humans, causing significant economic and production losses. The objective of this study was to determine the seroprevalence of F. hepatica in sheep from Boavita, Boyacá, Colombia. An observational, descriptive cross-sectional study was carried out with simple random sampling, where the sample size was 297 sheep. The sera were processed with the commercial ELISA BIO K 211-Monoscreen AbELISA F. hepatica kit, following the manufacturer's instructions. The analysis of the results was carried out with the statistical program Epi Info®. A general seroprevalence of 67.34% was determined, where males, sheep older than 3 years, and Creole sheep presented the highest seroprevalence, with 77.78, 83.33, and 64.79%, respectively. A significant statistical association was established between seropositivity to F. hepatica and sheep aged 1 to 3 and individuals older than 3 years. This age group was established as a risk factor for the manifestation of fasciolosis, whereas sheep between 1 to 3 years old were considered as a protective factor. Management variables such as extensive and stake grazing also showed statistical significance, the latter being a protective factor for parasitosis. Our findings show the spread of F. hepatica infections in sheep and confirm the importance of serological methods for the diagnosis of the disease.

Referencias

Acici, M., Buyuktanir, O., Bolukbas, C. S., Pekmezci, G. Z., Gurler, A. T., Umur, S. (2017). Serologic detection of antibodies against Fasciola hepatica in sheep in the middle Black Sea region of Turkey. Journal of Microbiology, Immunology and Infection, 50(3), 377-381. https://doi.org/10.1016/J.JMII.2015.06.005

Adamu, M., Wossene, A., Tilahun, G., Basu, A. K. (2019). Comparative diagnostic techniques in ruminant fasciolosis: Fecal sedimentation, indirect ELISA, liver inspection and serum enzyme activities. Ethiopian Veterinary Journal, 23(1), 42-58. https://doi.org/10.4314/evj.v23i1.4

Afshan, K., Qayyum, M., Rizvi, S. S. R., Mukhtar, M., Mushtaq, M., Miller, J. E. (2013). Serological and coprological comparison for rapid diagnosis of Fasciola hepatica infection in small ruminants from sub-tropical area of Pakistan. Small Ruminant Research, 113(1), 267-272. https://doi.org/10.1016/j.smallrumres.2013.01.020

Alcaldía de Boavita. (2020). Municipio de Boavita. Gobierno de Colombia. http://www.boavita-boyaca.gov.co/tema/municipio

Alvarez Rojas, C. A., Jex, A. R., Gasser, R. B., Scheerlinck, J.-P. (2014). Techniques for the diagnosis of Fasciola infections in animals: Room for improvement. In Advances in Parasitology (pp. 65-107). Academic Press. https://doi.org/10.1016/B978-0-12-800182-0.00002-7

Arifin, M. I., Höglund, J., Novobilský, A. (2016). Comparison of molecular and conventional methods for the diagnosis of Fasciola hepatica infection in the field. Veterinary Parasitology, 232, 8-11. https://doi.org/10.1016/j.vetpar.2016.11.003

Charlier, J., de Meulemeester, L., Claerebout, E., Williams, D., Vercruysse, J. (2008). Qualitative and quantitative evaluation of coprological and serological techniques for the diagnosis of fasciolosis in cattle. Veterinary Parasitology, 153(1-2), 44-51. https://doi.org/10.1016/j.vetpar.2008.01.035

Charlier, J., Duchateau, L., Claerebout, E., Williams, D., Vercruysse, J. (2007). Associations between anti-Fasciola hepatica antibody levels in bulk-tank milk samples and production parameters in dairy herds. Preventive Veterinary Medicine, 78(1), 57-66. https://doi.org/10.1016/j.prevetmed.2006.09.010

Charlier, J., Rinaldi, L., Musella, V., Ploeger, H. W., Chartier, C., Vineer, H. R., Hinney, B., von Samson-Himmelstjerna, G., Băcescu, B., Mickiewicz, M., Mateus, T. L., Martinez-Valladares, M., Quealy, S., Azaizeh, H., Sekovska, B., Akkari, H., Petkevicius, S., Hektoen, L., Höglund, J., Morgan, E.R., Bartley, D. J., Claerebout, E. (2020). Initial assessment of the economic burden of major parasitic helminth infections to the ruminant livestock industry in Europe. Preventive Veterinary Medicine, 182, e 105103. https://doi.org/10.1016/j.prevetmed.2020.105103

Charlier, J., Vercruysse, J., Morgan, E., van Dijik, J., Williams, D. L. (2014). Recent advances in the diagnosis, impact on production and prediction of Fasciola hepatica in cattle. Parasitology, 141(3), 326-335. https://doi.org/10.1017/S0031182013001662

Erdem, A., Simsek, S., Koroglu, E. (2012). A comparison of faecal examination, commercial ELISA kit, and indirect-ELISA methods in the diagnosis of sheep fasciolosis. Small Ruminant Research, 107(2-3), 164-166. https://doi.org/10.1016/j.smallrumres.2012.05.006

Fairweather, I. (2011). Reducing the future threat from (liver) fluke: Realistic prospect or quixotic fantasy? Veterinary Parasitology, 180(1-2), 133-143. https://doi.org/10.1016/J.VETPAR.2011.05.034

Fairweather, I., Brennan, G. P., Hanna, R. E. B., Robinson, M. W., Skuce, P. J. (2020). Drug resistance in liver flukes. International Journal for Parasitology: Drugs and Drug Resistance, 12, 39-59. https://doi.org/10.1016/j.ijpddr.2019.11.003

Figueiredo Marques, G., Pompei, J. C. A., Martini, M. (2017). Manual veterinario de toma y envío de muestras 2017. Panaftosa - OPS/OMS

Gao, X., Zhang, L., Tong, X., Zhang, H., Mehmood, K., Jiang, X., Li, J. (2020). Epidemiological survey of fasciolosis in yaks and sheep living on the Qinghai-Tibet plateau, China. Acta Tropica, 201, e105212. https://doi.org/10.1016/j.actatropica.2019.105212

Gayo, V., Cancela, M., Acosta, D. (2020). Maintenance of life cycle stages of Fasciola hepatica in the laboratory. In M. Cancela, G. Maggioli (Eds.), Fasciola hepatica: Methods and Protocols, Methods in Molecular Biology, 2137. Springer. https://doi.org/10.1007/978-1-0716-0475-5_1

Giraldo Pinzón, E., Pérez Cárdenas, J., Aguilar Marín, S., Linares Villalba, S. (2016). Prevalencia de fasciolosis bovina en una zona de Caldas Colombia con evidencias de la enfermedad. U.D.C.A. Actualidad & Divulgación Científica, 19(1), 149-154. https://doi.org/10.31910/rudca.v19.n1.2016.119

Hayward, A. D., Skuce, P. J., McNeilly, T. N. (2021). The influence of liver fluke infection on production in sheep and cattle: A meta-analysis. International Journal for Parasitology, 51(11), 913-924. https://doi.org/10.1016/j.ijpara.2021.02.006

Hu, R.-S., Zhang, X.-X., Ma, Q.-N., Elsheikha, H. M., Ehsan, M., Zhao, Q., Fromm, B., Zhu, X.-Q. (2021). Differential expression of microRNAs and tRNA fragments mediate the adaptation of the liver fluke Fasciola gigantica to its intermediate snail and definitive mammalian hosts. International Journal for Parasitology, 51(5), 405-414. https://doi.org/10.1016/j.ijpara.2020.10.009

Instituto Colombiano Agropecuario. (2019). Censo Pecuario año 2019. ICA. https://www.ica.gov.co/areas/pecuaria/servicios/epidemiologia-veterinaria/censos-2016/censo-2018

Joachim, A., Ali, S. Daugschies, A. (2002). Fasciola hepatica alters coagulation parameters in sheep plasma in vivo and in vitro. Parasitology Research, 89, 53-58. https://doi.org/10.1007/S00436-002-0723-3

Kamaludeen, J., Graham-Brown, J., Stephens, N., Miller, J., Howell, A., Beesley, N. J., Hodgkinson, J., Learmount, J., Williams, D. (2019). Lack of efficacy of triclabendazole against Fasciola hepatica is present on sheep farms in three regions of England, and Wales. Veterinary Record, 184(16), e105209. https://doi.org/10.1136/vr.105209

López Corrales, J., Cwiklinski, K., de Marco Verissimo, C., Dorey, A., Lalor, R., Jewhurst, H., McEvoy, A., Diskin, M., Duffy, C., Cosby, S. L., Keane, O. M., Dalton, J. P. (2021). Diagnosis of sheep fasciolosis caused by Fasciola hepatica using cathepsin L enzyme-linked immunosorbent assays (ELISA). Veterinary Parasitology, 298, e109517. https://doi.org/10.1016/j.vetpar.2021.109517

Mavrogianni, V. S., Papadopoulos, E., Spanos, S. A., Mitsoura, A., Ptochos, S., Gougoulis, D. A., Barbagianni, M. S., Kyriazakis, I., Fthenakis, G. C. (2014). Trematode infections in pregnant ewes can predispose to mastitis during the subsequent lactation period. Research in Veterinary Science, 96(1), 171-179. https://doi.org/10.1016/j.rvsc.2013.11.009

Mikaeel, F. B. (2020). Prevalence of Fasciola hepatica in goats and sheep by using ELISA in sera and milk in Duhok, Iraq. The Iraqi Journal of Veterinary Medicine, 44(2), 113-119. https://doi.org/10.30539/ijvm.v44i2.983

Munita, M. P., Rea, R., Martinez-Ibeas, A. M., Byrne, N., McGrath, G., Munita-Corbalan, L. E., Sekiya, M., Mulcahy, G., Sayers, R. G. (2019). Liver fluke in Irish sheep: Prevalence and associations with management practices and co-infection with rumen fluke. Parasites & Vectors, 12, e3779. https://doi.org/10.1186/s13071-019-3779-y

Pan, M., Bai, S.-Y., Ji, T.-K., Fan, Y.-M., Liu, D.-D., Yang, Y., Tao, J.-P., Huang, S.-Y. (2022). Epidemiology of Fasciola spp. in the intermediate host in China: A potential risk for fasciolosis transmission. Acta Tropica, 230, e106394. https://doi.org/10.1016/j.actatropica.2022.106394

Pérez-Creo, A., Díaz, P., López, C., Béjar, J., Martínez-Sernández, V., Panadero, R., Díez-Baños, P., Ubeira, F., Morrondo, P. (2016). Fasciola hepatica in goats from north-western Spain: Risk factor analysis using a capture ELISA. Veterinary Journal, 208, 104-105. https://doi.org/10.1016/j.tvjl.2015.07.033

Pinilla, J. C., Florez, A. A., Orlandoni, G., Tobón, J. C., Ortiz, D. (2020) a. Current status of prevalence and risk factors associated with liver fluke Fasciola hepatica in cattle raised in different altitudinal regions of Colombia. Veterinary Parasitology: Regional Studies and Reports, 22, e100487. https://doi.org/10.1016/j.vprsr.2020.100487

Pinilla, J. C., Florez, A. A., Uribe Delgado, N. (2020) Prevalence and risk factors associated with liver fluke Fasciola hepatica in cattle and sheep in three municipalities in the Colombian Northeastern Mountains. Veterinary Parasitology: Regional Studies and Reports, 19, e100364. https://doi.org/10.1016/j.vprsr.2019.100364

Pinilla León, J. C., Uribe Delgado, N., Florez, A. A. (2019). Prevalence of gastrointestinal parasites in cattle and sheep in three municipalities in the Colombian Northeastern Mountain. Veterinary World, 12(1), 48-54. https://doi.org/10.14202/vetworld.2019.48-54

Pritchard, G. C., Forbes, A. B., Williams, D. J. L., Salimi-Bejestani, M. R., Daniel, R. G. (2005). Emergence of fasciolosis in cattle in East Anglia. Veterinary Record, 157(19), 578-582. https://doi.org/10.1136/VR.157.19.578

Pulido Villamarín, D. P., Castañeda Salazar, R., Arbeláez, G. (2011). Fasciola hepatica: pedagogía de diagnóstico por laboratorio y su situación en Colombia. Revista Electrónica de Veterinaria, 12(5B)

Recalde-Reyes, D. P., Padilla Sanabria, L., Giraldo Giraldo, M. I., Toro Segovia, L. J., González, M. M., Castaño Osorio, J. C. (2014). Prevalencia de Fasciola hepatica, en humanos y bovinos en el departamento del Quindío-Colombia 2012-2013. Infectio, 18(4), 153-157. https://doi.org/10.1016/j.infect.2014.09.001

Regea, G., Getachew, G. (2021). Study on prevalence and associated risk factors of ovine fasciolosis in and around Nekemte Town, Oromia, Ethiopia. Journal of Veterinary Medicine and Animal Sciences, 4(1). https://meddocsonline.org/journal-of-veterinary-medicine-and-animal-sciences/Study-on-prevalence-and-associated-risk-factors-of-ovine-fasciolosis-in-and-around-nekemte-town-oromia-ethiopia.pdf

Reichel, M. P. (2002). Performance characteristics of an enzyme-linked immunosorbent assay for the detection of liver fluke (Fasciola hepatica) infection in sheep and cattle. Veterinary Parasitology, 107(1-2), 65-72. https://doi.org/10.1016/S0304-4017(02)00095-X

Rokni, M. B. (2014). Helminth-Trematode: Fasciola hepatica and Fasciola gigantica. In Encyclopedia of Food Safety (pp. 140-145). Elsevier. https://doi.org/10.1016/B978-0-12-378612-8.00154-2

Vercruysse, J., Claerebout, E. (2001). Treatment vs non-treatment of helminth infections in cattle: Defining the threshold. Veterinary Parasitology, 98(1-3), 195-214. https://doi.org/10.1016/S0304-4017(01)00431-9

Cómo citar

APA

Bulla-Castañeda, D.-M., Ortiz-Pineda, M.-C., Cruz-Estupiñan, S.-E., Lopez-Buitrago, H.-A., Lancheros-Buitrago, D.-J., Giraldo-Forero, J.-C., Garcia-Corredor, D.-J., y Pulido-Medellin, M.-O. (2023). Diagnóstico serológico de Fasciola hepatica en ovinos de Boavita (Colombia). Revista Científica, 46(1), 27–36. https://doi.org/10.14483/23448350.19724

ACM

[1]
Bulla-Castañeda, D.-M., Ortiz-Pineda, M.-C., Cruz-Estupiñan, S.-E., Lopez-Buitrago, H.-A., Lancheros-Buitrago, D.-J., Giraldo-Forero, J.-C., Garcia-Corredor, D.-J. y Pulido-Medellin, M.-O. 2023. Diagnóstico serológico de Fasciola hepatica en ovinos de Boavita (Colombia). Revista Científica. 46, 1 (ene. 2023), 27–36. DOI:https://doi.org/10.14483/23448350.19724.

ACS

(1)
Bulla-Castañeda, D.-M.; Ortiz-Pineda, M.-C.; Cruz-Estupiñan, S.-E.; Lopez-Buitrago, H.-A.; Lancheros-Buitrago, D.-J.; Giraldo-Forero, J.-C.; Garcia-Corredor, D.-J.; Pulido-Medellin, M.-O. Diagnóstico serológico de Fasciola hepatica en ovinos de Boavita (Colombia). Rev. Cient. 2023, 46, 27-36.

ABNT

BULLA-CASTAÑEDA, D.-M.; ORTIZ-PINEDA, M.-C.; CRUZ-ESTUPIÑAN, S.-E.; LOPEZ-BUITRAGO, H.-A.; LANCHEROS-BUITRAGO, D.-J.; GIRALDO-FORERO, J.-C.; GARCIA-CORREDOR, D.-J.; PULIDO-MEDELLIN, M.-O. Diagnóstico serológico de Fasciola hepatica en ovinos de Boavita (Colombia). Revista Científica, [S. l.], v. 46, n. 1, p. 27–36, 2023. DOI: 10.14483/23448350.19724. Disponível em: https://revistas.udistrital.edu.co/index.php/revcie/article/view/19724. Acesso em: 31 ene. 2023.

Chicago

Bulla-Castañeda, Diana-María, Melissa-Camila Ortiz-Pineda, Sharon-Elizabeth Cruz-Estupiñan, Henrry-Alexander Lopez-Buitrago, Deisy-Johana Lancheros-Buitrago, Julio-Cesar Giraldo-Forero, Diego-Jose Garcia-Corredor, y Martin-Orlando Pulido-Medellin. 2023. «Diagnóstico serológico de Fasciola hepatica en ovinos de Boavita (Colombia)». Revista Científica 46 (1):27-36. https://doi.org/10.14483/23448350.19724.

Harvard

Bulla-Castañeda, D.-M., Ortiz-Pineda, M.-C., Cruz-Estupiñan, S.-E., Lopez-Buitrago, H.-A., Lancheros-Buitrago, D.-J., Giraldo-Forero, J.-C., Garcia-Corredor, D.-J. y Pulido-Medellin, M.-O. (2023) «Diagnóstico serológico de Fasciola hepatica en ovinos de Boavita (Colombia)», Revista Científica, 46(1), pp. 27–36. doi: 10.14483/23448350.19724.

IEEE

[1]
D.-M. Bulla-Castañeda, «Diagnóstico serológico de Fasciola hepatica en ovinos de Boavita (Colombia)», Rev. Cient., vol. 46, n.º 1, pp. 27–36, ene. 2023.

MLA

Bulla-Castañeda, D.-M., M.-C. Ortiz-Pineda, S.-E. Cruz-Estupiñan, H.-A. Lopez-Buitrago, D.-J. Lancheros-Buitrago, J.-C. Giraldo-Forero, D.-J. Garcia-Corredor, y M.-O. Pulido-Medellin. «Diagnóstico serológico de Fasciola hepatica en ovinos de Boavita (Colombia)». Revista Científica, vol. 46, n.º 1, enero de 2023, pp. 27-36, doi:10.14483/23448350.19724.

Turabian

Bulla-Castañeda, Diana-María, Melissa-Camila Ortiz-Pineda, Sharon-Elizabeth Cruz-Estupiñan, Henrry-Alexander Lopez-Buitrago, Deisy-Johana Lancheros-Buitrago, Julio-Cesar Giraldo-Forero, Diego-Jose Garcia-Corredor, y Martin-Orlando Pulido-Medellin. «Diagnóstico serológico de Fasciola hepatica en ovinos de Boavita (Colombia)». Revista Científica 46, no. 1 (enero 2, 2023): 27–36. Accedido enero 31, 2023. https://revistas.udistrital.edu.co/index.php/revcie/article/view/19724.

Vancouver

1.
Bulla-Castañeda D-M, Ortiz-Pineda M-C, Cruz-Estupiñan S-E, Lopez-Buitrago H-A, Lancheros-Buitrago D-J, Giraldo-Forero J-C, Garcia-Corredor D-J, Pulido-Medellin M-O. Diagnóstico serológico de Fasciola hepatica en ovinos de Boavita (Colombia). Rev. Cient. [Internet]. 2 de enero de 2023 [citado 31 de enero de 2023];46(1):27-36. Disponible en: https://revistas.udistrital.edu.co/index.php/revcie/article/view/19724

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